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PCR Methylation specific PCR

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A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA DNA gel extraction / PCR product purification Product size > 15Kb

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Get tips on using QuantiTect SYBR Green RT-PCR Kit (1000) to perform PCR Conventional / Qualitative PCR - mammalian DNA

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Get tips on using High Pure PCR Product Purification Kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Products Roche Lifesciences High Pure PCR Product Purification Kit

Get tips on using Agencourt AMPure XP - PCR Purification to perform

Products Beckman Coulter Agencourt AMPure XP - PCR Purification

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